Journal: Chromosoma

Article Title: CTCF is essential for proper mitotic spindle structure and anaphase segregation.

doi: 10.1007/s00412-023-00810-w

Figure Lengend Snippet: Fig. 4 Mitotic spindle structure is perturbed in CTCF knock- downs. A Example images of mitosis in wild-type and CTCF knockdowns c13 and c21 with labeled microtubules (green, α-tubulin) and DNA (magenta, Hoechst). Mitotic spindles were classified as abnormal mitotic spindle if they were tri-/ tetrapolar spindles (yellow) or other abnormal (blue) if DNA was substantially behind the spindle pole or no division was observed. For all images, see Supplemental Figure 1. B Graph of the percentage of abnormal mitotic spindles (WT n = 0/48; CTCF knockdown c13 n = 12/42; CTCF knockdown c21 n = 12/57; CENP-E inhibitor GSK923295 72 h n = 11/17). C Box and whisker plot of average and individual measurement of DNA position relative to the spindle pole. Purple dots represent spindles where DNA is > 1 μm behind the spindle pole (WT 0%, c13 30%, c21 12%, GSK923295 29%). Error bar represents standard error. Statistical tests are Student’s t-tests, significance denoted by *p < 0.05, **p < 0.01, and ***p < 0.001, while no signifi- cance is denoted by ns. Scale bar is 10 μm

Article Snippet: Coverslips were placed on 50-μL drops of the primary antibody mixture consisting of rat anti-alpha tubulin at 1:100 dilution (Novus biological); mouse anti-lamin A/C at 1:100 dilution (Cell Signaling Technology); and PBS with 5% BSA.

Techniques: Labeling, Knockdown, Whisker Assay

Journal: The Febs Journal

Article Title: Identification and verification of heat shock protein 60 as a potential serum marker for colorectal cancer

doi: 10.1111/j.1742-4658.2011.08385.x

Figure Lengend Snippet: Western blot qualification of differentially expressed protein spots. (A) 2D-DIGE image and corresponding 3D simulation of the HSP60 spot in a matched tissue sample. N, normal tissue; T, tumoral tissue. (B) Relative expression of HSP60, GST-Pi, TCP1β, CK19 and HSP90β in paired CRC samples analyzed by 2D-DIGE. Relative expression corresponds to the spot volume determined with imagemaster 2d-platinium software, transformed into logarithm base 2, and normalized with the corresponding spot volume of the internal standard image (Cy2). Comparisons were performed with the Wilcoxon signed-rank test. (C) Western blot analysis of protein expression in eight independent tissue sample pairs. Tubulin was used as loading control.

Article Snippet: Antibodies against α-enolase (sc-100812), PGAM1 (sc-130334), TCP1β (sc-28556) and HSP90β (sc-69703) were from Santa-Cruz Biotechnology (Heidelberg, Germany), antibody against α-tubulin (clone 17H11) was from Rockland Immunochemicals (Gilbertsville, PA, USA), and antibody against CK19 (61010) was from Progen (Heidelberg, Germany).

Techniques: Western Blot, Expressing, Software, Transformation Assay, Control